Cloning of type 1 pili pilA gene from avian Escherichia coli

鸡源大肠杆菌1型菌毛结构基因（pilA）克隆

Sequencing and bioinformation analysis of type I pili pilA gene of pathogenic Escherichia coli isolated from ducks

鸭源致病性大肠杆菌Ⅰ型菌毛pilA基因序列测定及生物信息学分析

The Study of Anti-Fimbriae of Type 1 from Avian E.Coli and the Fusion Expression of the Pila Gene and the Immunogenic Study of the Recombinant Fimbriae Proteins

抗鸡大肠杆菌的1型菌毛的单克隆抗体的研究和pilA基因的融合表达及其重组菌毛蛋白免疫原性研究

The immunoprotection effect of pilA recombinant protein vaccine was evaluated according to the mortality , re-isolated rate of E. coli , and grades of pathological changes .

coli分离率和各组织器官的病变等级来判定pilA重组蛋白的免疫保护效果。

We also find the C-terminus of PilA possesses cysteine residues that are conserved in type ⅳ pilus of many species , and it forms a disulphide bond that produces a loop , which is important for the function of pilus and also comprises a major antigenic determinant . 2 .

另外，PilA蛋白羧基端拥有相对保守的半胱氨酸残基，该位点能够形成二硫键并产生一个环形结构，在Ⅳ型菌毛发挥功能时起到重要作用，而且是主要的抗原决定部位。